Mouse IgG3 binding to macrophage-like cells is prevented by deglycosylation of the antibody or by Accutase treatment of the cells

Alicja Karabasz,Tomasz Klaus,Joanna Bereta,Maria Czarnek,Monika Bzowska,Maja Sochalska

doi:10.1038/s41598-021-89705-3

Alicja Karabasz, Tomasz Klaus + Show 4 more

Open Access

https://doi.org/10.1038/s41598-021-89705-3

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Abstract

The binding of mouse IgG3 to Fcγ receptors (FcγR) and the existence of a mouse IgG3-specific receptor have been discussed for 40 years. Recently, integrin beta-1 (ITGB1) was proposed to be a part of an IgG3 receptor involved in the phagocytosis of IgG3-coated pathogens. We investigated the interaction of mouse IgG3 with macrophage-like J774A.1 and P388D1 cells. The existence of an IgG3-specific receptor was verified using flow cytometry and a rosetting assay, in which erythrocytes clustered around the macrophage-like cells coated with an erythrocyte-specific IgG3. Our findings confirmed that receptors binding antigen-free IgG3 are present on J774A.1 and P388D1 cells. We demonstrated for the first time that the removal of N-glycans from IgG3 completely abolished its binding to the cells. Moreover, we discovered that the cells treated with Accutase did not bind IgG3, indicating that IgG3-specific receptors are substrates of this enzyme. The results of antibody-mediated blocking of putative IgG3 receptors suggested that apart from previously proposed ITGB1, FcγRII, FcγRIII, also additional, still unknown, receptor is involved in IgG3 binding. These findings indicate that there is a complex network of glycan-dependent interactions between mouse IgG3 and the surface of effector immune cells.

Highlights

The binding of mouse IgG3 to Fcγ receptors (FcγR) and the existence of a mouse IgG3-specific receptor have been discussed for 40 years
J774A.1 and P388D1 cells express a receptor for Mouse IgG3 (mIgG3)
A competitive blockade of FcγRI and FcγRIV with mouse IgG2a, a highaffinity ligand of these receptors, reduced M18-488 binding only when IgG2a was used at a concentration equal or higher than that of mIgG3 (Fig. 3 and Supplementary Fig. S10). These results indicate that FcγRII, FcγRIII and integrin beta-1 (ITGB1) are involved in the binding of free mIgG3

Summary

Introduction

The binding of mouse IgG3 to Fcγ receptors (FcγR) and the existence of a mouse IgG3-specific receptor have been discussed for 40 years. The results of antibody-mediated blocking of putative IgG3 receptors suggested that apart from previously proposed ITGB1, FcγRII, FcγRIII, additional, still unknown, receptor is involved in IgG3 binding. These findings indicate that there is a complex network of glycan-dependent interactions between mouse IgG3 and the surface of effector immune cells. FcγRs are divided into high-affinity receptors, which bind free IgGs, and low-affinity receptors, which bind IgGs complexed with a polyvalent antigen. It was shown that on the surface of mouse immune cells, including macrophages, there is an unidentified receptor that binds m IgG34–6 This unknown receptor mediates the recognition of pathogens opsonized with m IgG3s5,6. Flow cytometry analysis confirmed that the affinity of IgG3 to FcγRI is low[7]

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