Motor units in the human medial gastrocnemius muscle are not spatially localized or functionally grouped.

Abstract

Human medial gastrocnemius (MG) motor units (MUs) are thought to occupy small muscle territories or regions, with low-threshold units preferentially located distally. We used intramuscular recordings to measure the territory of muscle fibres from MG MUs and determine whether these MUs are grouped by recruitment threshold or joint action (ankle plantar flexion and knee flexion). The territory of MUs from the MG muscle varied from somewhat localized to highly distributed, with approximately half the MUs spanning at least half the length and width of the muscle. There was also no evidence of regional muscle activity based on MU recruitment thresholds or joint action. The CNS does not have the means to selectively activate regions of the MG muscle based on task requirements. Human medial gastrocnemius (MG) motor units (MUs) are thought to occupy small muscle territories, with low-threshold units preferentially located distally. In this study, subjects (n=8) performed ramped and sustained isometric contractions (ankle plantar flexion and knee flexion; range: ∼1-40%maximal voluntary contraction) and we measured MU territory size with spike-triggered averages from fine-wire electrodes inserted along the length (seven electrodes) or across the width (five electrodes) of the MG muscle. Of 69 MUs identified along the length of the muscle, 32 spanned at least half the muscle length (≥6.9cm), 11 of which spanned all recording sites (13.6-17.9cm). Distal fibres had smaller pennation angles (P<0.05), which were accompanied by larger territories in MUs with fibres located distally (P<0.05). There was no distal-to-proximal pattern of muscle activation in ramp contraction (P=0.93). Of 36 MUs identified across the width of the muscle, 24 spanned at least half the muscle width (≥4.0cm), 13 of which spanned all recording sites (8.0-10.8cm). MUs were not localized (length or width) based on recruitment threshold or contraction type, nor was there a relationship between MU territory size and recruitment threshold (Spearman's rho=-0.20 and 0.13, P>0.18). MUs in the human MG have larger territories than previously reported and are not localized based on recruitment threshold or joint action. This indicates that the CNS does not have the means to selectively activate regions of the MG muscle based on task requirements.