Motion-picture phase imaging by an integrated optical system of a parallel phase-shifting digital holographic microscope

Abstract

A parallel phase-shifting digital holographic microscope achieves motion-picture phase imaging of a dynamic minute specimen. However, much time and skill are required to construct the optical system of the microscope. The authors designed and constructed an optical system of a parallel phase-shifting digital holographic microscope in which the optical components of the microscope were integrated on a breadboard standing perpendicular to an optical table. The lateral and longitudinal magnifications of the microscope are 10 and 100, respectively. The authors experimentally demonstrated the motion-picture phase imaging of a dynamic minute specimen by the microscope. The holograms were recorded at 1,000 fps and the shutter speed was 0.5 ms.