Motile activity and cytoskeleton changes in uveal melanoma after proton beam radiation

Abstract

PurposeProton beam irradiation is known to invoke different biological response than photons.We compared the effect of proton beam irradiation and X‐rays on the survival and migratory properties of melanoma cells.MethodsMel270, human uveal melanoma cell line was treated with physical doses of 1‐5 Gy of proton beam (58 MeV) from Proteus C‐235 cyclotron or X‐rays (300 kV Phillips) at the dose rate of 1 Gy/min were given to suspended cells.ResultsInvasive potential of cells was slightly diminished after both types of radiation at 20 days post‐radiation and then returned to normal at 40 days. Wound healing ability was decreased after X‐rays. The beta‐1‐integrin level was decreased after both types of radiation in Mel270 cells, and vimentin, a marker of EMT was not changed. Less stress fibers were seen in cytoskeleton of Mel270 cells after radiation.ConclusionsLow doses of both types of radiation influenced the motile activity and cytoskeleton in Mel270 cells.