Abstract
AIMSTo assess the cellular proliferation using the monoclonal antibody Ki-67, in paraffin embedded uveal melanomas irradiated by proton beam, as well as in non-irradiated uveal melanomas.METHODS30 enucleated eyes were included...
Highlights
We undertook an immunohistochemical study using anti-Ki-67 antibody to assess the proliferation rate of a series of uveal melanomas treated by proton beam irradiation, and we compared our findings with the Ki-67 immunoreactivity of non-irradiated uveal melanomas
Monoclonal antibodies against PCNA (PC10) have been reported to assess cell proliferation in conventionally fixed histological material.[6 13 14 18 25]
Since PCNA is detectable in almost quiescent cells adjacent to some tumours[26] presumably because of its long half life, and since its prognostic value is contested,[6 18 25] we have studied the proliferation activity in irradiated and non-irradiated uveal melanomas using the Ki-67 immmunostaining
Summary
Forty consecutive patients with uveal melanoma underwent enucleation between 1991 and 1996, either without adjunct therapy (17 patients) or after proton beam irradiation (23 patients). Eyes were enucleated if the tumour size was excessive (maximum tumour height >10 mm) or in the case of neovascular glaucoma. Enucleation was performed in cases of continued tumour growth or tumour recur-. The mitotic figures were counted in 40 high power fields (HPF) and a quantitative evaluation of balloon cells was performed in 10 HPFs. rence, or in cases of neovascular glaucoma. Nine irradiated eyes were excluded from this study for the following reasons: association of brachytherapy before proton beam irradiation (three cases), technically unsatisfactory samples (paraYn blocks not available for the preparation of histological sections, four cases), or extensively necrotic tumours (two cases). The following pretreatment clinical data included: age and sex of the patient, delay of the irradiation after diagnosis, echographic maximum tumour height (MTH) and largest tumour diameter (LTD), and the location of the tumour. Each stained section was evaluated, on 20 random HPFs, with count repeated three times and averaged
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