Abstract
Three populations, two from Colombia and one from Brazil, of Rotylenchulus reniformis associated with banana and plantain, were characterized using morphological, morphometric, and molecular methods. Morphometric data from these populations were similar to type and reference populations of R. reniformis. Partial sequences of both D2-D3 rDNA and mitochondrial cytochrome oxidase subunit I (COI) regions had a strong affinity (99% similarity) to previously published sequences of R. reniformis. Phylogenetic analyses (maximum likelihood and Bayesian inference) suggested that the Colombian populations of R. reniformis corresponded to the previously described Type A of the species. This is the definitive first report in Colombia of R. reniformis associated with banana and plantain crops.
Highlights
Second only to black sigatoka disease caused by Mycosphaerella fijiensis Morelet (Araya, 2003), phytonematodes are considered the most limiting factor of Musa spp production in Colombia
To address this knowledge gap, the present study aims to: identify by morphological, morphometric, and molecular analysis the species of Rotylenchulus associated with Musa spp. in Colombia, and analyze the phylogenetic relationship of Rotylenchulus species
The Colombian and Brazilian populations analyzed in this study were identified morphologically and morphometrically as R. reniformis (Table 1 and Fig. 1)
Summary
Second only to black sigatoka disease caused by Mycosphaerella fijiensis Morelet (Araya, 2003), phytonematodes are considered the most limiting factor of Musa spp production in Colombia. Rotylenchulus is a semi-endoparasitic and sedentary phytonematode that is of economic importance throughout (sub-)tropical and temperate zones reducing yield and quality of almost 150 crop species (Castillo and Gómez-Barcina, 1993; Robinson et al, 1997; Crozzoli et al, 2004; Khan, 2005; Moore and Lawrence, 2012; Jones et al, 2013). While numerous reports of Rotylenchulus associated with Musa spp. in Colombia exist (Zuñiga et al, 1979; Barriga and Cubillos, 1980; Curiel and Ospino, 2001; Gómez, 2001; Guzmán et al, 2012), detailed morphological, morphometric, and molecular data were not included. In Colombia, there is limited knowledge as to which species of Rotylenchulus are associated with Musa spp. which impedes the deployment of effective management strategies to control the species.
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