Abstract
World patchouly oil need is almost 90% fulfilled by Indonesia as the main producer, however, their narrow genetic variation is a major obstacle in plant breeding. Chemical mutation such as colchine is believed to be able to wider patchouly genetic variation The aim of this study was to increase patchouly genetic variation using chemical mutagen. The variety of Patchoulina 2 was treated by colchicine on callus stage. Callus irradiated was grown into regeneration medium until formed plantlet and ready to acclimatization. The results showed that there were 15 different morphological putative mutants with different characters. The mutants varied in qualitative characters(leaf shape, leaf color, leaf base, and leaf edge)and quantitative characters(plant height, number of leaf stalks). Two distinguishable main groups of mutants shared 62.62% genetic similarity level based on morphology. These new mutants in this study would be potential to be developed as new varieties along with the morphological characters as their specific identities.
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