Abstract
ELECTRON microscope studies of the virus producing a chronic respiratory disease in turkeys prevously reported by the authors describe the virus as having an average mean diameter of 60 millimicrons with oblong heads and filamentous projections which seem to be flatter than the projections observed in bronchitis virus (Reagan et al., 1948).The virus of the chronic respiratory disease in chickens used in these studies was isolated by one of the authors (J. P. Delaplane). The virus was subjected to 26 serial passages in 10-day embryonated eggs before use in electron microscope studies. Approximately 25 percent of the embryos of the 26th passage were dead or appeared sluggish within 96 to 168 hours. The allantoic fluid from these eggs was harvested and filtered through a Type ST size L3 Seitz filter. The filtered virus suspension was then subjected to centrifugation in a Spinco Ultracentrifuge at 44,400 r.p.m. for 2 hours, .
Published Version
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