Abstract
Genus Salix is among family Salicaceae, distributing in the northern hemisphere. It is represented in Egypt by two species (Salix mucronata and Salix tetrasperma). The classification of Salix at the generic and infra-generic levels is still outstanding. We have agreed to list the Egyptian species of this genus. We collected them during field trips to most Egyptian habitats; fresh and herbarium specimens were subjected to taxonomic revision based on morphological characters; scanning electron microscope (SEM) for pollen grains; isozyme analysis using esterase and peroxidase enzymes and genetic diversity using random amplified polymorphic DNA (RAPD). We recorded that both sexes of S. mucronata existed but there were only male trees from S. tetrasperma. SEM of pollen grain revealed that there were many differences between them. Also, the application of isozyme analysis giving 12 sex indicating bands. In addition, ten RAPD primers retrieved a total of 227 amplified bands (77 for female S. mucronata, 74 for male S. mucronata and 76 for male S. tetrasperma).
Highlights
Genus Salix Linnaeus (1753: 1015) of Salicaceae, is known as willows commonly [1]
On the other hand, [2] revealed that the classification of Salix at the generic and infrageneric levels is still unresolved; the essential taxonomic difficulties of the genus Salix are too increased by their high phenotypic plasticity and great variation in diagnostic morphological characters In Egypt it is represented by two species; Salix tetrasperma Roxb., Salix safsaf Trautv. (Latter treated as Salix mucronata Thunb.)
Because molecular markers have so many advantages over morphological markers, we decided to classify the Egyptian species of this genus using random amplified polymorphic DNA (RAPD) and isozymes markers in addition to pollen grain characters
Summary
Genus Salix Linnaeus (1753: 1015) of Salicaceae, is known as willows commonly [1] It is moisture loving trees and shrubs, with long lanceolated leaves, it is planted around banks to hold soil in suburban yards [2]. As an important genetic marker, it could be used to detect the inheritance and variance among different species or different cultivars at the molecular level It could be as a biochemical marker to study the tolerance of plant to stress [14]. Because molecular markers have so many advantages over morphological markers, we decided to classify the Egyptian species of this genus using RAPD and isozymes markers in addition to pollen grain characters
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