Morphological features of epithelial cells in the sheep isthmus oviduct during early pregnancy.

Abstract

Listen

Translate article

The objective of the present study was to define the morphological changes that occur in the epithelium of the isthmus oviduct of the sheep during the first few weeks of pregnancy. Isthmus oviducts were obtained from ovariectomized, estrous (day O) and pregnant (day 2, 3, 4, 6, and 16) ewes and analyzed using light and electron microscopy. The epithelium was low cuboidal in the isthmus from ovariectomized sheep, significantly increased in height at estrus, underwent an additional increase at day 3, and was significantly reduced by day 4 of pregnancy. Ciliated and nonciliated epithelial cells were present at all reproductive stages. Ciliated cells were always the predominant cell type. The percentage of ciliated and nonciliated cells did not significantly change at any stage examined. In addition, a third cell type, located adjacent to the basement membrane, was present. These "basal" cells were rounded in appearance and more frequently observed in the epithelial lining of ovariectomized ewes and after day 3 of pregnancy. The secretory organelles of nonciliated epithelial cells obtained from ovariectomized ewes were poorly developed. In estrous ewes, the Golgi apparatus in nonciliated cells consisted of stacked cisternae that atrophied later in pregnancy. The most striking alterations in the secretory apparatus occurred in ciliated cells. Two populations of ciliated cells were observed at estrus-one with abundant, membrane bound supranuclear granules and one without granules. The granules in ciliated cells were rare after day 3 of pregnancy and no evidence of secretion was ever observed. Large lipid droplets were present in the cytoplasm of ciliated cells at estrus. Lysosomes were abundant in nonciliated cells by day 16 of pregnancy. Regularly arranged microvilli were present in nonciliated cells obtained from ovariectomized, estrous, and pregnant ewes. No appreciable change in nuclear shape or chromatin content was observed at any reproductive stage examined. The ultrastructural characteristics and relative abundance of other cytoplasmic organelles such as the RER, SER, mitochondria, and glycogen varied slightly during pregnancy in ciliated and nonciliated cells. These data show that the epithelial lining of the isthmus oviduct in the sheep undergoes subtle, yet distinctive, structural changes during the first few weeks of pregnancy.