Morphological changes of ischemic myocardial injury induced by sodium laurate

Abstract

Objective To observe the morphological changes of myocardium and intracoronary microthrombus by intracoronary injection of sodium laurate. Methods Fifty four male SD rats, weighing (250 ± 30)g,were randomly divided into 3 groups according to weight: sodium laurate group, sham group and control group,eighteen rats in each group. In sodium laurate group, exposed and occluded the aorta while injected with sodium laurate(1 mg/kg) for 20 seconds. In sham group, took normal saline instead and no operation in control group. At the 1st, 7th and 28th day after the operation, 4 - 6 rats were selected randomly from each group and euthanized. The cardiac tissue were obtained and fixed for pathological study. After HE staining and modified Lendrum staining,micro-thrombosis and morphological changes of injured myocardium were detected under light microscope. The ultrastructure change of vascular endothelial cells was observed with transmission electron microscopy (TEM).Results Microthrombus consisting of platelet fibers and collagen could be observed only in sodium laurate group.The ultrastroctural changes of endothelial cells were significant in sodium laurate group compared with other two groups. TEM analysis showed that tight junctions between cells became wide and microvascular became narrowed in sodium laurate group. The fibrosis changes were enhanced in sodium laurate group compared with sham group and control group with the time prolong. Conclusions Micro-thrombosis is one of mechanisms in the development of sodium laurate induced ischemic myocardial injury. Endothelial injury may be the mechanism for sodium laurate leading to microthrombus. Key words: Myocardial ischemic; Sodium laurate; Coronary thrombosis; Histology,comparative