Abstract
The objective of this study was to identify morphological variation in M2 plants of Capsicum annuum derived from seed treated with ethyl methanesulfonate (EMS). The M1 generation was developed by treated seed with 0.5%, 0.75% and 1% EMS in phosphate buffer pH 7.0 for 6 hours. Seedlings of M1 were planted in the field and seeds resulted from M1 plants were harvested and planted for morphological analysis. Plants were planted in polybag in progeny row system. Results showed that there was a decrease in both seedling emergence and plant survival due to EMS treatments. Several morphological variations were observed in plant height, leaf size, pattern of the branch, number of main stems and petal number of the flower. In the M2 generation, treatment of 1% EMS generated tall plant, small plant with pale green leaf colour, dwarf plant mutant and plant with two stems. The 0.75% EMS resulted in the short mutant with many branches while 0.5% EMS produced plant with pale green leaf colour. These results indicated that EMS mutagenesis in C. annuum generated interesting morphological characters that differ to control plants which can be used in C. annuum improvement program.
Highlights
Capsicum annuum L. is one of the horticultural plants that have high economic value in Indonesia
This study aimed to evaluate ethyl methane sulfonate (EMS) concentrations that induce mutation in chili pepper and to analyze the morphological variations induced by EMS at M2 generation
The effect of EMS to plant variation and growth was influenced by the amount of mutagen uptake or part of embryo affected by EMS18.High concentration of EMS inhibited physiological process for seed germination including inhibition of catalase and lipase activity, hormone imbalance and mitosis inhibition which lead to poor growth[19]
Summary
Capsicum annuum L. (chili pepper) is one of the horticultural plants that have high economic value in Indonesia. (chili pepper) is one of the horticultural plants that have high economic value in Indonesia. It mainly used daily as a food ingredient, pickle or as materials for a food industry. Besides that, it is known as one component of herbal. In Indonesia, the productivity of chili pepper shows high fluctuation. It was reported that in 2015, the productivity of chili pepper was 1.045.182 ton, while in 2016 the productivity increased sharply to 10.205.694 ton[2]. Several factors contributed to the productivity of chili pepper such as high pest and disease, climate change, as well as the used of a low quality of chili pepper seed cultivars
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
