Abstract
Ethylmethane sulfonate (EMS) mutagenesis is a powerful hunting tool to seek novel players for improving agromonic traits. Together with rapid evolution of the next-generation sequencing techniques, the EMS mutagenesis has been revaluated for its utilization to breed crops in practical agriculture and to study functions of key players in valuable agronomic traits. In this study, we systematically investigated conditions for EMS mutagenesis in Dongjin (Oryza sativa, Japonica) rice plants to make a mutant population. Since the EMS mutagenesis depends on target tissue, EMS concentration and EMS exposure time, we fixed the EMS exposure time as 13 h and treated germinating seeds with various levels of EMS dosage (from 0.25 to 2% EMS concentration). EMS treatment clearly showed negative biological influences including low germination and abnormal seedling development of Dongjin rice plants. Based on the standard of about 50% lethal dose, 0.75 and 1% EMS dosage for 13 h was finally selected as the optimal conditions for EMS mutagenesis of Dongjin germinating seeds.
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