Morphological Changes in The Collagen Matrix During the Subcutaneous Implantation of Fibrous Polymer Frame

Ruslan Kozovyi

doi:10.26717/bjstr.2020.25.004236

Abstract

The study included 20 laboratory animals (rabbits) divided into 2 groups: 10 animals of Group I underwent surgery involving the creation of a subcutaneous pocket and suturing; 10 animals of Group II underwent subcutaneous implantation of the biopolymer matrix into the back area between the shoulder blades. Nine segments were used for the study, namely 1 centrally located segment, 4 segments of the paracentral zone, 4 segments of the peripheral zone. Statistical analysis of the results was conducted using software programs Microsoft Excel and Statistica 5.5 (Multiple Regression) by means of the methods of variation statistics, correlation.

Highlights

The major challenge in tissue engineering is to optimize cell isolation, multiplication and differentiation, as well as to construct the matrices or delivery systems thereby promoting the maintenance and coordination of three-dimensional tissue regeneration
Nine segments were used for the study, namely 1 centrally located segment, 4 segments of the paracentral zone, 4 segments of the peripheral zone
The fibrous matrix constructed by us creates a kind of the bridge for tissue ingrowth and the formation of a three-dimensional collagen matrix

Summary

Introduction

The major challenge in tissue engineering is to optimize cell isolation, multiplication and differentiation, as well as to construct the matrices or delivery systems thereby promoting the maintenance and coordination of three-dimensional tissue regeneration. One of the important criteria that should be considered when constructing the matrix is its ability to form an optimal scaffold for the transplantation of the cell substrates. Biopolymers are currently attracting increased attention in medicine and bioengineering. The major challenge in tissue engineering is to optimize cell isolation, multiplication and differentiation, as well as to construct the matrices or delivery systems thereby promoting the maintenance and coordination of three-dimensional tissue regeneration [2,3]. One of the important criteria that should be considered when constructing the matrix is its ability to form an optimal scaffold in combination with optimal hemodynamics inside the scaffold to transplant the cell substrates [4,5]. The aim of our study was to experimentally assess the nature of the development of collagen fibers during all the periods of subcutaneous implantation of the biopolymer fibrous matrix

Objectives

Methods

Results

Discussion

Conclusion