Morphological changes in the broiler chickens liver at use of “Karsilin” in conditions of infectious-toxic hepatitis modelling

Abstract

In poultry farms, at the conditions of rapid intensification of production, a lot of stress factors, such as infectious, technological, fodder, medication and others, act on the bird organism, and the main burden is borne by the liver. Therefore, timely warning and control of liver disease is very important. One of the marker infections with a potent pathogenic toxic effect on the liver and the bird organism as a whole is pseudomonosis. Objective: study of morphological changes in the liver of broiler chickens at the use of “Karsilin” in the conditions of modeling of infectious-toxic hepatitis for the determination of hepatoprotective action. We conducted a histological examination of the liver of intact chickens (intact control); chickens infected with the strain of P. aeruginosa microorganisms (control pathology); chickens, for which the “Karsilin” or Thiotriazolin was used with preventive-therapeutic purposes at infecting P. aeruginosa. Microscopic picture of liver samples infected with P. aeruginosa chicks corresponds to moderately expressed non-specific reactive hepatitis with morphological signs: disorganization of the hepatic beams pattern, hydrocytic and fatty hepatocyte dystrophy, small foci necroses, inflammatory cellular infiltration; proliferative, stagnant processes, perivascular edema and hyperemia in the system of the hepatic triad. The use of “Karsilin” at experimental pseudomonosis reduced the intensity of pathological processes: the severity of hepatocyte dystrophy was less than 71%, and necrotic changes in hepatocytes and violations of the beam pattern were less than 75% compared with the control pathology. Feed supplement “Karsilin” demonstrates hepatoprotective effect, which is expressed by preventing or significantly reducing the intensity of the manifestation of dystrophic, necrotic, infiltrative and proliferative processes in hepatocytes and in the hepatic triad in conditions of infectious-toxic hepatitis caused by the pathological action of P. aeruginosa toxins in the body of chicken broilers. Feed supplement “Karsilin” on the activity of protective and regenerative action is not inferior, and according to many indicators, the comparison preparation Thiotriazolin prevails.