Morphological and photosynthetic responses of riparian plant Distylium chinense seedlings to simulated Autumn and Winter flooding in Three Gorges Reservoir Region of the Yangtze River, China

Abstract

To evaluate the tolerance of riparian plant Distylium chinense in Three Gorges Reservoir Region to anti-season flooding, a simulation flooding experiment was conducted during Autumn and Winter, and morphology and photosynthesis of D. chinense seedlings and their recovery growth after soil drainage were analyzed in different duration of flooding and flooding depth. The seedlings were submitted to four treatments: (1) 40 seedlings unflooded and watered daily as control (Unflooded, CK); (2) 120 seedlings flooded at 1 cm above the ground level (F-1 cm); (3) 120 seedlings flooded at 12 cm above the ground level (F-12 cm) and (4) 120 seedlings completely submerged with 2 m water depth (F-2 m, top of plants at 2 m below water surface). The flooding survival, plant height, stem diameter, adventitious roots, stem lenticels, epicormic shoots, chlorophyll content and photosynthesis parameters were determined at 0, 15, 30, 90 days in flooding stress and 15, 60 days after soil drainage. The results showed that the survival of the seedlings subjected to flooding was 100% for all repeated measurements in all treatments. Adventitious roots, hypertrophied lenticels and stem hypertrophy were observed in the seedlings flooded for more than 15 d, and increased with the prolonged flooding duration, while disappeared after the soil was drained. Flooding duration and flooding depth showed significant individual and interactive effects on leaf chlorophyll a (Chl a), chlorophyll b (Chl b), and their ratio, chlorophyll ( a + b), the net photosynthesis rate ( P n ), transpiration rate ( T r ), stomatal conductance ( C s ), and inter-cellular CO 2 concentration ( C i ) of D. chinense seedlings ( P < 0.01). After 15 days of flooding, there was no significant decrease in P n of the flooded seedlings as compared with that of the control seedlings. P n of the flooded seedlings was significantly lower than that of the control seedlings after 30 days of flooding ( P < 0.05), whereas P n showed no significant difference among seedlings from three flooding depths. After 90 days of flooding, P n of the F-2 m flooded seedlings was significantly lower than that of the controls, F-1 cm and F-12 cm flooded seedlings ( P < 0.05), but still maintained high photosynthetic capacity. P n of the F-1 cm and F-12 cm flooded seedlings rose gradually after soil drainage, while, it was significantly lower than that of the control seedlings after 15 days of recovery ( P < 0.05). After 60 days of recovery, P n of all seedlings flooded with different depths showed no significant difference as compared with that of the control seedlings and new leaves grew out in the F-2 m flooded seedlings. The effect of all flooding treatments on G s , T r , Chl a, Chl b, Chl a/Chl b and chl (a + b) was basically the same as their effect on P n , while the effect of all flooding treatments on C i was quite the contrary. Correlation analysis showed that P n was positively relative with G s , T r , Chl a, Chl b and chl (a + b) ( P < 0.05) and significantly negative with C i ( P < 0.05). Therefore, the present study demonstrates that D. chinense has high survival and good recovery growth after long-term flooding in anti-season flooding and could be taken as an excellent candidate species in the re-vegetation of water-level-fluctuation areas in Three Gorges Reservoir Region.