Abstract
An anisakid nematode larva found in cod sold in the state of Minas Gerais, Brazil was studied by light and scanning electron microscopy and by a molecular approach. Mitochondrial cytochrome c-oxidase subunit 2 (mtDNA cox-2), 28S rRNA and ITS1, 5.8S and ITS2 regions were amplified using the polymerase chain reaction and sequenced to evaluate the phylogenetic relationships of the larva. The genetic profile confirmed that this larva belongs to the species Pseudoterranova decipiens (sensu stricto). This is the first molecular and ultrastructural study of Pseudoterranova decipiens (sensu stricto) in imported cod sold in Brazil. The health implications of these findings are discussed.
Highlights
Fishes known as cod comprise five different species
An anisakid nematode larva found in cod sold in the state of Minas Gerais, Brazil was studied by light and scanning electron microscopy and by a molecular approach
For the 28S gene in relation to P. decipiens, there was a 100% identity, 99% query cover and a maximum score of 1382; no match was found for P. azarasi
Summary
Fishes known as cod comprise five different species. The term ‘cod’ originally referred to fishes from the Atlantic and Pacific Oceans that are processed by salting and drying (OLIVEIRA et al, 2012). Among these species, Gadus morhua Linnaeus, 1758 and Gadus macrocephalus Tilesius, 1810 (Gadidae) are known as Atlantic and Pacific cod that occur, respectively, in the North Atlantic and Pacific Oceans.
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