Abstract
Research on the analysis of morphological and genetic variations of Batak fish (Tor soro) in North Sumatra has been conducted. The Batak fish samples were captured from flowing rivers at several districts in North Sumatera namely: Bahorok River (Langkat), Alian River (Toba Samosir), Batang Toru River (South Tapanuli). Truss method was used to analyze morphological parameters by using SPSS ver. 16.0 computer program, while the genetic parameters were analyzed by scoring amplified DNA strands visualized in electrophoresis using Polymerase Chain Reaction – Random Amplified Polymorphism DNA (PCR-RAPD) method. Primers used in this study were: OPA-02, OPA-03, OPA-04, OPC-01 and OPC-02. The PCR amplicons were then analyzed by using Numerical Taxonomy and Multivariate Analysis System (NTSYS) ver. 2.02. computer program. Dendrogram analysis showed that the level of morphological variability was at 1–25 euclidean distance. Dendogram analysis also showed that genetic diversity level was within coefficient of 0.23–0.71 in which obtained a diversity level of 48%. Based on the morphological and genetic results, it may be indicated that the Batak fishes originating from Batang Toru had a close similarity with Alian’s rather than Bahorok’s.
Published Version
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