Abstract
Objective: To investigate the influence of salt mine medium from the Romanian Cacica and Dej Salt Mines upon the cell morphology and electrophoretic expression of heterogeneous skin cell cultures obtained from Wistar rats’ abdominal skin, on normal and Ovalbumin-sensitized animals. Materials and methods: Heterogeneous skin cell cultures were prepared from Wistar abdominal skin. Cultures derived from skin rat develop with a monolayer of fibroblasts and epithelial cells attached to the culture dish. Before cultures initiation, Wistar rats of 75-100 g weight were divided in two lots: control and ovalbumin challenged animals. Ten animals of each lot were send to Cacica and Dej Salt Mine for 14 days and maintained in the salt mine medium, as in speleotherapy treatment. Results: Speleotherapy of Wistar rats had induced significant differences in cell morphology and electrophoretic expression of primary dermal cells cultures. The data obtained support the protective effects of speleotherapy by comparing with ovalbumin sensibilised animals. Conclusions: The results of this study indicate the fact that speleotherapy induces changes on the morphology and protein expression of dermal cells in vitro, and these changes support the beneficial effects of speleotherapy.
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