Abstract
Objective: To investigate the influence of Cacica and Dej salt mines microclimate on the morphology and electrophoresis protein expression of in vitro dermal fibroblasts from hypodermic tissue of Wistar rats in normal conditions and after experimental induced wounds and burns. Materials and methods: Dermal fibroblast cultures were obtained from hypodermic tissue collected from Wistar rats. Grow in cultures derived fibroblast monolayer is attached to the cultivation flask. Before initiation of cultures, Wistar rats weighing 75-100 g were separated into three groups: control, wounds and burns. Five animals from each group were sent to Cacica and Dej salt mines, for 14 days and kept in saline environment, as in speleotherapy. Results: Speleotherapy applied to Wistar rats caused significant differences in cell morphology and electrophoretic expression of dermal fibroblasts in primary culture completed. The data obtained confirm the therapeutic effects of speleotherapy compared with experimental data from control animals. Conclusions: The results of this study indicate that speleotherapy induces changes in morphology and protein expression of in vitro dermal fibroblasts and therapeutic effects of these changes are present in the wounds and burns cases.
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