Abstract
The article gives the results of the research on the influence of a developed complex liposomal preparation on the dynamics of morphological and biochemical indicators of the blood of rats subjected to modeled oxidative stress caused by intermuscular injection of 50% carbon tetrachloride at a dose of 0.25 ml per 100 g of body mass. We found that in conditions of intoxication by carbon tetrachloride, the physiological level of hematological indicators of the researched animals’ body was disrupted. This is indicated by the reduction in the amount of erythrocytes, hemoglobin content, concentration of hemoglobin per erythrocyte, increase in the amount of leucocytes, mass of hemoglobin per erythrocyte and increase of colour indicator. Also, we detected suppression of protein synthesis function of the liver. The levels of total protein and albumin fraction were below the normal physiological level. High indicators were observed in the levels of creatinine, urea and total bilirubin. In order to normalize the functional state of the liver under oxidative stress it is reasonable to use a liposomal preparation which contains butafosfan, interferon, thistle and vitamins. In order to normalize morphological and biochemical indicators of the blood of rats subject to intoxication of carbon tetrachloride it is reasonable to use a liposomal preparation that contains butafosfan, selenium, thistle, methionine and vitamins. Using the liposomal preparation Butaselmevit for rats under oxidative stress the morphological and biochemical indicators in the blood normalized. On the 14th day the indictors of the number of erythrocytes, the hemoglobin content, the white blood cell count and red blood indexes compared to control came within the physiological indicators, indicating a recovery of hematopoietic function of the bone marrow and normalized indicators of the functional state of the liver.
Highlights
The intensive introduction of chemical preparations into industry and agriculture, deterioration of the environment, pollution of the environment with xenobiotics, uncontrolled usage of medicines cause an increase of toxic effects in human and animal organisms (Li еt al., 2012; Gutyj еt al., 2017)
As it is shown in the table, in rats of the research group D1 poisoned with carbon tetrachloride the amount of erythrocytes decreased by 32.7% and the level of hemoglobin in blood decreased by 18.2%
The hematopoietic function of bone marrow is violated in rats subject to intoxication with carbon tetrachloride, which can be observed in the decrease in the amount of erythrocytes by 34%, content of hemoglobin by 18%, concentration of hemoglobin per erythrocyte by 30%, increase of the mass of hemoglobin per erythrocyte by 38%, increase in volume of erythrocyte by 77%, increase in colour indicator by 42%
Summary
The intensive introduction of chemical preparations into industry and agriculture, deterioration of the environment, pollution of the environment with xenobiotics, uncontrolled usage of medicines cause an increase of toxic effects in human and animal organisms (Li еt al., 2012; Gutyj еt al., 2017). It is known that the central organ which conducts the detoxification processes of organisms is the liver (Calabrese еt al., 1999; Cherkashina and Petrenko, 2006). The role in pathogenesis of liver diseases played by processes of activation of free radical oxidation of lipids (FROL) plasmatic and intracellular membranes of hepatocytes against the background of the depletion of protective antiradical systems has been determined (Gutyj еt al., 2016). As a result of metabolism of ССl4, products of a free radical nature which are inductors of lipid peroxidation (POL) are created in the organism, as a result of which the structure of liver cells and their major functions is disrupted (Calabrese еt al., 1999; Chen еt al., 2000; Cherkashina and Petrenko, 2006; Vyshtakaliuk еt al., 2015)
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