Changes in the morphometry of the uterus, ovary, and foetus, and biochemistry of allantoic and amniotic membrane fluids of Yankasa ewes across the gestation period.

Abstract

This study evaluated the uterine and fetal morphometric changes and fetal membrane fluids biochemistry across the gestation of Yankasa sheep. The amniotic and allantoic fluids are actively involved in the constant physiologic exchange between the fetus and maternal circulation. Hence, the knowledge regarding changes in the composition of fetal membrane fluids is important for understanding fetal metabolism, and the diagnosis of pathophysiological conditions during gestation. Gravid uteri from 37 ewes and their corresponding ovaries were sampled. The number and size of the placentomes in the second and third terms of gestation were significantly higher relative to the first term. The total protein, albumin, glucose, urea, creatinine, and calcium levels as well as alkaline phosphatase (ALP) and aspartate aminotransferase (AST) activities were higher in the allantoic fluid. In the allantoic fluid, the levels of total protein, globulin, and the activity levels of ALT and AST increased progressively with the advancement of gestation; contrarily, the levels of calcium, chloride, and the activity level of ALT decreased. For the amniotic fluid, the levels of total protein, globulin, urea, calcium, and the enzyme activities in the second and third terms did not differ but were higher than the level in the first term of gestation. In addition, the most significant increases in creatinine level and white blood cell count were observed in the third term of gestation. Therefore, notable differences in the levels of ALT, AST, total proteins, glucose, urea, creatinine, and WBC counts were observed in the two fetal membrane fluids.