Abstract
Immature embryo- and anther-derived suspension and protoplast cultures were established in hexaploid tritordeum. The morphogenic capability of these cultures was studied under different nitrogen regimes. Both embryo- and anther-derived suspensions showed morphogenic capability. Suspension and protoplast cultures established from immature embryos gave rise to green shoots and green plants, whereas albino plants were regenerated when anther cultures were used as suspension-starting material. Chromosomal stability of suspensions and regenerated plants was analysed, revealing considerable instability in these cultures.
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