Abstract
Tiliacora racemosa is a climbing shrub belonging to the family Menispermaceae and is used in traditional medicine for treating different ailments such as snakebites, cuts and wounds, strangury and also used as a diuretic. The morphoanatomical studies help to identify diagnostic features for the identification and standardization of the medicinal plant. The plant material was fixed, sectioned using rotary microtome and stained with toluidine blue. The leaf consists of very thick midrib with small lateral veins both being plano-convex. The midrib consists of three top or egg shaped collateral vascular bundles which are surrounded by a common sclerenchymatous bundle sheath and the lamina is dorsiventral. The adaxial epidermis is apostomatic and the abaxial epidermis is stomatiferous which is of cyclocytic type. The stem is circular in sectional view and consists of about 19 discrete wedge shaped vascular bundles. The narrow and wide fibres, vessel elements were seen in powder microscopy. Wide fibres, vessel elements were seen in powder microscopy.
Highlights
India is rich in traditional knowledge and traditional healers use different parts of medicinal plants as medicine [1]
The stomata are of cyclocytic type which is in agreement with the data obtained from the previous studies of the plant [22] whereas anomocytic stomata were observed by Kundu and Guha [23]
Macroscopical studies revealed that Tiliacora racemosa has simple leaves with alternate phyllotaxy, ovate to lanceolate, acuminate at apex, obtuse or cordate at base, glabrous with undulate margin
Summary
India is rich in traditional knowledge and traditional healers use different parts of medicinal plants as medicine [1]. The major drawback to the use of herbal medicine is the lack of standardization. This paves way for wrong identification, unintended substitution of closely related species and intended adulteration of genuine drugs with quality less ones to meet the increasing demands of the herbal drugs [2]. After 24 hrs of fixing, the specimens were dehydrated with graded series of tertiary–butyl alcohol [15]. Infiltration of the specimens was carried by gradual addition of paraffin wax (melting point 58-60 C) until tertiary–butyl alcohol solution attained super saturation.
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