Abstract
An investigation into the role of carbohydrates in flowering of the endangered species Kniphofia leucocephala Baijnath. (Asphodelaceae) in vitro revealed that a carbohydrate source is essential for the induction of inflorescences. Both the concentration and type of sugar influenced the percentage of flowering, with 60 g l−1 fructose and 10 μM N6-benzyladenine (BA) inducing the best flowering response. A high percentage of flowering was also observed with 60 g l−1 glucose and 10 μM BA. The optimal concentration of sucrose for flower induction was 30 g l−1, beyond which the flowering percentage declined, but could be partially restored by increasing the BA concentration. Although overall plant growth declined at high sugar concentrations, there does not appear to be any significant correlations between various growth parameters and flowering percentage, suggesting that sugars play a direct role in floral transition in vitro.
Published Version
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