Abstract
When embryonic stem cells are differentiated in the presence of activin A in serum-free conditions, an endoderm progenitor population defined by the coexpression of either Brachyury, Foxa2 and c-Kit, or c-Kit and Cxcr4 is generated. Specification of these progenitors with bone morphogenetic protein-4 in combination with basic fibroblast growth factor and activin A results in the development of hepatic populations highly enriched (45–70%) for cells that express the alpha-fetoprotein and albumin proteins.
Highlights
Setup T0: Differentiation is performed at 5% O2, 5% CO2
Detailed differentiation media recipe is attached in the page
Summary
2. Setup T0: Differentiation is performed at 5% O2, 5% CO2.
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