Monofloral Triadica Cochinchinensis Honey Polyphenols Improve Alcohol-Induced Liver Disease by Regulating the Gut Microbiota of Mice.

Liping Luo,Wenjie Yu,Jinping Zhang,Fangjian Ning,Tao Liu,Mingyan Liu,Shengrong Qiu,Shengxiang Yi,Xueyong Huang

doi:10.3389/fimmu.2021.673903

Abstract

Honey produced from medicinal plants holds great promise for human health. Increasing evidence suggests that the gut microbiota plays an important role in liver pathology after alcohol intake. The aim of this study was to identify the polyphenol composition of triadica cochinchinensis honey (TCH), and to study the potential effect of honey polyphenols on the regulation of gut microbes in mice with alcohol-induced liver injury and the improvement of alcohol-induced liver disease. For these purposes, a total of 190 compounds were identified and 27 of them were quantified by ultraperformance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-Q/TOF-MS) and we successfully established a mouse model of alcohol-induced liver injury. The results show that TCH polyphenols can significantly restore the levels of ALT and AST, and TCH intervention can significantly improve the pathological changes of liver tissue in alcohol-exposed mice. Additionally, a significant decrease was observed in Firmicutes/Bacteroidetes after TCH treatment. Moreover, KEGG pathways of ATP-binding cassette (ABC) transporters, two-component system and biosynthesis of amino acids enriched the most differentially expressed genes after TCH intervention for 8 weeks. Our results may have important implications for the use of TCH as a functional food component with potential therapeutic utility against alcohol-induced liver disease.

Highlights

Alcoholic liver disease (ALD) is a type of alcohol-induced chronic progressive liver disease [1]
BAJAJ et al proposed to reflect the changes of “good bacteria” and “bad bacteria” in the intestinal flora of patients with the liver disease through the cirrhotic flora imbalance index. This ratio is calculated by dividing the number of beneficial intestinal tracts of Lachnospiraceae, Ruminococcaceae, and Clostridiales Family XIV Incertae sedis by the number of potentially pathogenic bacteria Enterobacteriaceae and pseudobacteriaceae
Other studies have found that, Verrucomicrobia, Actinobacteria, Corynebacterium, Proteobacteria, and Proteobacteria are rich in the intestines of alcoholic mice.The fecal Veillonellaceae and Megasphera of patients with alcoholic hepatitis and liver cirrhosis increased significantly

Summary

Introduction

Alcoholic liver disease (ALD) is a type of alcohol-induced chronic progressive liver disease [1]. Alcohol-related liver cirrhosis accounts for 0.9% of global deaths and 47.9% of all deaths from cirrhosis-related diseases, which seriously threatens human health [2]. The progression of ALD includes multiple stages, from steatohepatitis, alcoholic hepatitis, liver fibrosis, cirrhosis, and even liver cancer [3]. At this stage, there is still a lack of effective drug treatments for ALD. The possible mechanisms of ALD are mainly alcohol metabolism-related oxidative stress damage, abnormal methionine metabolism, intestinal flora imbalance and bacterial translocation, inflammatory mediator damage, and nutritional imbalance, etc.

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