Abstract
Bifunctional antibodies, expressing specificity for both hepatitis B surface antigen (HBsAg) and prostatic acid phosphatase (PAP), were secreted by a hybridoma produced by a modified cell fusion procedure. A hybridoma making mouse monoclonal antibody to PAP was back selected and fused with spleen cells from a mouse immunized with HBsAg. Clones were isolated which secreted antibody capable of binding both antigens simultaneously. Antibody secreted by one such hybridoma could be separated into three well defined species by DEAE chromatography. Trace-labeled antigen binding experiments clearly demonstrated that one was the original antibody specific for only PAP, another a new antibody derived from a spleen cell specific for only HBsAg, and the third a hybrid antibody with specificity for both PAP and HBsAg expressed in the same molecule. More than half of the antibody molecules secreted by the hybridoma were present in this hybrid form. Similar antibodies, with one specificity for a tumor associated antigen and the second for a toxin, drug, or radioactive reagent should be useful for in vivo immunodiagnostics and immunotherapeutics.
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