Abstract
Confocal laser scanning microscopy (CLSM) may be used either as fluorescence or reflectance microscopy,1 with much of the previous work in human skin focusing on the investigation of pigmented2 and non-pigmented skin malignancies.3,4 Until now there has been little published on CLSM analysis of skin microvasculature in vivo, although there has been some work assessing dermal capillaries in fluorescent antibody-labelled skin biopsy specimens.5 Therefore the authors are to be congratulated on carrying out this early study demonstrating that reflectance CLSM has the potential to be a valuable bedside analysis tool for free flap monitoring.
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