Abstract
Our aim was to study the selected cases of the patients with ischemic heart disease and to analyze the structure of blood serum of patients in comparison with control serum of healthy subjects by methods: synchronous fluorescence fingerprint and atomic force microscopy that are still not used in clinical practice. Our results of fluorescence analysis showed that blood serum of all patients with ischemic heart disease decreased intensity of fluorescence in comparison with control blood serum. Endogenous fluorescence of synchronous fluorescence fingerprint of blood serum of patients with unstable angina pectoris state after non ST elevation myocardial infarction; angina pectoris and arterial hypertension 3 was similar, but atomic force microscopy revealed differences in the structure of blood serum of patients with the angina pectoris. Blood serum of patients with angina pectoris exhibited disappearance of fluorescence peak with maximum fluorescence and showed lower fluorescence intensity than control blood serum and blood serum of patients with arterial hypertension 2. Profiles of synchronous fluorescence fingerprint of blood serum of patients with arterial hypertension stage 2 showed formation of the new fluorescent peak with maximum fluorescence, similar shape of synchronous fluorescence fingerprint and higher fluorescence intensity than blood serum of healthy subjects. Blood serum sensitively revealed changes in the body by using untraditional novel techniques which enable the analysis of the mixture of blood serum and might be a new possibility in study of heart ischemia diseases.
Highlights
Myocardial ischemia can lead to damage of the heart muscle, reducing its ability to pump efficiently and lead to the serious abnormal heart rhythms
Graphical comparison of the 3-D maps (Figure 1) and the contour maps (Figure 2, Figure 3) of synchronous fluorescence fingerprint (SFF) of blood serum of healthy subjects is a profile with a characteristic shape differing from patients with heart ischemia
Our results showed decreased intensity of fluorescence of blood serum of all patients with heart ischemia in comparison with control blood serum
Summary
Myocardial ischemia can lead to damage of the heart muscle, reducing its ability to pump efficiently and lead to the serious abnormal heart rhythms. The patients with myocardial ischemia exhibited higher serum levels of glucose and lactate and concomitant lower serum levels of fatty acids [4]. Oxidative stress is the state of increased production of reactive oxygen species (ROS) which lead to oxidative modification of proteins and lipoproteins [13]. It is a potential hallmark of ischemia diseases. It is important to understand and to differentiate the normal physiological variations of ischemia and angiogenesis and to differentiate from the pathological changes This experimental work studied ischemic heart diseases in blood serum of patients with various representative cases of cardiovascular disease. This work can contribute to study an early detection of ischemic heart disease by using untraditional methods of fluorescence spectroscopy and atomic force microscopy, which are not used in clinical practice
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