Abstract
Barto‰ikova L ., J . N eaas, V . S uch˘, R . K ubinova, D . V esela, L . B ene‰, T. Barto‰ik, J. Illek, J. ·alplachta, J. Klusakova, L. Barto‰ova, V. Strnadova, P. FraAa, J. FraAova: Monitoring of Antioxidative Effect of Morine in Alloxan-induced Diabetes mellitus in the Laboratory Rat. Acta Vet. Brno 2003, 72: 191-200. The purpose of the study was to monitor the antioxidative effect of morine in alloxan-induced diabetes mellitus in laboratory rat. The animals were divided by random selection into two groups (n = 7). The treated group was given morine in oral doses of 10 mg/kg -1 in 0.5% solution of Methocel E5 once a day; the control diabetic group was given only the solution of Methocel E5. Once a week, selected laboratory indices were determined in all animals (glucose, urea and cholesterol levels in serum, total glucose and protein losses through urine); diuresis as well as antioxidative enzymes (superoxiddismutase, glutathione peroxidase); total antioxidative capacity and malondialdehyde level in the blood. On the 20th day the animals were exsanguinated and kidney tissue and pancreas samples were taken for histopathological analysis. We found a significant increase (p ≤ 0.05) of the glutathione peroxidase catalytic activity in the treated group compared to control diabetic group. There was also a highly significant increase (p ≤ 0.01) of total antioxidative capacity in the treated group compared to control diabetic group. A significant decrease (p ≤ 0.05) of malondialdehyde level was identified in the treated group compared to the control diabetic group. The superoxiddismutase catalytic activity involved nonsignificant changes. A significant decrease (p ≤ 0.05) of cholesterol level in serum was identified in the treated group compared to control diabetic group. Other examined laboratory parameters did not exhibit significant changes. Biochemical indices followed in this study indicated a protective antioxidative effect of morine. However, the results of histopathological examination did not correlate with them. Free radicals, morine, diabetes mellitus, malondialdehyde, antioxidative enzymes
Highlights
The purpose of the study was to monitor the antioxidative effect of morine in alloxan-induced diabetes mellitus in laboratory rat
Once a week, selected laboratory parameters were determined in all the animals by spectrophotometry using Diagnostika Lachema laboratory testing sets, as well as antioxidative enzymes and total antioxidative capacity by COBAS MIRA S automatic analyser, using RANDOX Dublin Ireland comp. testing sets
Diabetes mellitus is a syndrome initially characterized by a loss of glucose homeostasis
Summary
The purpose of the study was to monitor the antioxidative effect of morine in alloxan-induced diabetes mellitus in laboratory rat. We found a significant increase (p ≤ 0.05) of the glutathione peroxidase catalytic activity in the treated group compared to control diabetic group. A significant decrease (p ≤ 0.05) of malondialdehyde level was identified in the treated group compared to the control diabetic group. A significant decrease (p ≤ 0.05) of cholesterol level in serum was identified in the treated group compared to control diabetic group. Increased production of reactive forms of oxygen and lipoperoxides together with lowered levels of substances having antioxidative action (vitamin C, vitamin E, lipoic acid, glutathione) and enzymes acting as antioxidative agents, such as superoxiddismutase, catalase (Giugliano et al 1996; Pa cker et al 1995; Wohaieb and Godin 1987) can be found in the blood and tissues of patients affected with the disease. According to current views the antioxidative stress plays a significant role in the etiopathogenesis of diabetes of both 1st and 2nd types and has key importance in the occurrence and development of diabetic complications
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