Abstract
To evaluate DNA fragmentation and GMO quantification during soya bean protein concentrate and isolate preparation, genetically modified soya bean event GTS 40-3-2 (Roundup ReadyTM soya bean, RRS) was blended with conventional soya beans at mass percentages of 0.9%, 2%, 3%, 5% and 10%. Qualitative PCR and real-time PCR were used to monitor the taxon-specific lectin and exogenous cp4 epsps target levels in all of the main products and by-products, which has practical significance for RRS labelling threshold and traceability. Along the preparation chain, the majority of DNA was distributed in main products, and the DNA degradation was noticed. From a holistic perspective, the lectin target degraded more than cp4 epsps target during both of the two soya bean proteins preparations. Therefore, the transgenic contents in the final protein products were higher than the actual mass percentages of RRS in raw materials. Our results are beneficial to the improvement of GMO labelling legislation and the protection of consumer rights.
Highlights
Since the genetically modified organism (GMO) was commercially released, the cultivation of GMOs and their application in food and feed products has become increasingly widespread [1]
We discovered the distortions of RRS proportions in products obtained during soya bean protein preparation, which has barely been focused on before
A scale of raw soya beans containing 0.9%–10% (w/w) RRS were used for preparing soya bean protein concentrate (SPC) and soya bean protein isolate (SPI)
Summary
Since the genetically modified organism (GMO) was commercially released, the cultivation of GMOs and their application in food and feed products has become increasingly widespread [1]. With the consumption of GMO products, transgenic DNA and protein originating from GMO are likely to enter the human food chain [3], increasing the consumer concern about the potential hazards of GMO products [4]. To regulate the circulation of GMO products in the international market and help consumers make informed choices about foods that may contain GMO, many countries have established rules regarding the labelling of GMO products [5,6]. The rules in Japan, Thailand, Taiwan, South Africa and Indonesia state that any food product should be clearly labelled if it contains 5% or more GMO components [7]. The corresponding threshold values are 3% in South Korea [8] and 0.9% in the European Union and Russia [9]
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