Monitoring 5-fluorouracil temperature-sensitive hydrogel interstitial chemotherapy for pancreatic cancer with endoscopic ultrasonography

Abstract

Objective To evaluate the therapeutic effect of PLGA-PEG-PLGA-5- fluorouracil temperature -sensitive hydrogel interstitial chemotherapy for pancreatic cancer by using endoscopic ultrasonogra- phy. Methods PLGA-PEG-PLGA-5-fluorouracil temperature-sensitive hydrogel in vitro release experiments were performed in the following procedures： determination of lixivium drug concentration and calculation of its emission . Fifty nude mice with the pancreatic cancer cell line SW1990 were randomly divided into 5 groups, 10 in each group. Group A was intratumorally injected with PLGA-PEG-PLGA-5-tluorouraeil temper- ature-sensitive hydrogel at 4mg/kg ; group B with PLGA-PEG-PLGA-5-fluorouracil temperature-sensitive hy- drogel at 1 mg/kg; Group C was intratumorally injected with 5-fluorouracil at 4 mg/kg ; Group D with PLGA- PEG-PLGA matrix at 4mg/kg ;and group E was the control group. Tumor growth and audio-visual images of the nude mice tumor nodules were observed before administration , and 3,7,10,14 days after. Tumor growth curve was also drawn. Animals were sacrificed at 14 days Tumors were weighed to calculate the inhibitory rate and stained for pathological study. Results 1,3,5,8,10,and 14-day release of 5-fluorouracil temperature-sensitive hydrogel were 21.6% , 33.8 % , 44.3% , 63.6% , 76.3% and 91.8% . Tumor sizes of group A and group B were significantly different from those of other groups （ P 〈0. 05 ）. Ultrasound endoscopic image characteristics were correlated with pathological findings. Conclusion PLGA-PEG-PLGA-5- fluorouracil temperature-sensitive hydrogel is able to release for 14 days in vitro , which constantly inhibits human pancreatic cancer cell line SW1990 . lntratumoral injection of the agent can significantly inhibit the growth of pancreatic cancer of nude mice . Additionally, gelatinous preparations fixes better than liquid and is of clinical value. Therefore, monitoring temperature-sensitive 5-fluorouracil hydrogel interstitial chemotherapy for pancreatic cancer with endoscopic ultrasound is convenient and safe . Key words: Paucreatie cancer; Fluorouracil; Thermo-sensitive hydrogel; Endoscopic uhrasunography; Nude mouse