Molekularna identifikacija vrsta Mycobacterium tuberculosis kompleksa i netuberkuloznih mikobakterija

Abstract

Conclusion: Identification of mycobacteria isolated from respiratory specimens over a three-month period by using molecular techniques revealed that the most frequently isolated mycobacterial species in Serbia is M. tuberculosis, while nontuberculous mycobacteria (NTM) species isolated from respiratory specimens of local patients are M. gordonae, M. xenopi and M. kansasii. Introduction: Fast and accurate identification of Mycobacterium tuberculosis complex (MTBC) and nontuberculous mycobacteria to the species level is of great importance. Highly sensitive and specific molecular techniques are methods of choice for identification of mycobacteria to the level of species. The Aim: The aim of the study was identification of mycobacteria recovered from pulmonary specimens in Serbia during a three-month period, to the level of species. Material and methods: Identification of 112 mycobacterial cultures was performed using the Genotype MTBC and CM (HAIN, Lifescience, Germany) assays. Results: In total, 88 (78,57%) isolates were identified as species M. tuberculosis, while 24 (21,43%) isolates were identified as NTM. The identification to the species level was achieved in 17 (70,83%) NTM isolates, while 7 (29,17%) were identified as Mycobacterium sp. The most frequently isolated NTM species was M. gordonae (10; 58,82%), while the isolation rates of the remaining species were as follows: M. xenopi (5; 29,41%) and M. kansasii (2; 11,77%).