Molecularly Imprinted Polymer (MIP)-Based Electrochemical Sensor for Determination of Amyloid β-42 in Alzheimer’s Disease

Abstract

Alzheimer’s disease is the most common type of dementia caused by degeneration of the brain that affects a person’s ability to function independently. Amyloid beta 42 (Aβ- 42) is used as a biomarker for Alzheimer’s disease detection. Increasing the sensitivity of early stage detection of Alzheimer’s disease is challenging. This study mainly focused on the development of signal amplification of Aβ-42 detection using Graphene (G) and Carbon Dot (CD) which were modified on Screen-Printed Carbon Electrode (SPCE) to form graphenecarbon dot/ SPCE. The successful of modified SPCE was investigated by Cyclic Voltammetry (CV). The Molecularly Imprinted Polymer (MIP) was used for specific detection of Aβ- 42. The MIP-based Aβ-42 sensor was prepared by polymerization of o-phenylenediamine (oPD) monomer jointly with Aβ-42 template on the modified SPCE using the Differential Pulse Voltammetry (DPV) technique. The results showed the optimum conditions for the MIP fabrication; an electropolymerization cycle of 15 cycles and elution time of 8 minutes. The MIP-based Aβ-42 sensor exhibited the detection range of Aβ-42 from 1 to 30 pg/ml with the linear range from 0.5 to 20 pg/ml, the R2 from the regression curve of 0.9732 and a Limit of Detection (LOD) of 0.104 ng/ml. The developed MIPbased Aβ-42 sensor was suitable for Aβ-42 detection with cost effectiveness, high sensitivity, and easy to use.