Abstract
EV-B93 is a novel serotype within the Enterovirus B species and is uncommon worldwide. Currently, only one full-length genomic sequence (the prototype strain) has been deposited in the GenBank database. In this study, three EV-B93 were identified, including one from an acute flaccid paralysis (AFP) patient (named 99052/XZ/CHN/1999, hereafter XZ99052) and two from healthy children (named 99096/XZ/CHN/1999 and 99167/XZ/CHN/1999, hereafter XZ99096 and XZ99167, respectively) from Tibet in 1999 during the polio eradication program. The identity between the nucleotide and amino acid sequences of the Tibet EV-B93 strain and the EV-B93 prototype strain is 83.2%–83.4% and 96.8%–96.9%, respectively. The Tibet EV-B93 strain was found to have greater nucleotide sequence identity in the P3 region to another enterovirus EV-B107 as per a phylogenetic tree analysis, which revealed that recombination occurred. Seroepidemiology data showed that EV-B93 has not produced an epidemic in Tibet and there may be susceptible individuals. The three Tibet EV-B93 strains are temperature-resistant with prognosticative virulence, suggesting the possibility of a potential large-scale outbreak of EV-B93. The analyzed EV-B93 strains enrich our knowledge about this serotype and provide valuable information on global EV-B93 molecular epidemiology. What is more, they permit the appraisal of the serotype's potential public health impact and aid in understanding the role of recombination events in the evolution of enteroviruses.
Highlights
In order to investigate the similarities in genetic characteristics of EV-B93 and other EV-B strains, phylogenetic trees based on the entire VP1, P1, P2, and P3 coding regions were performed among the three Tibet EV-B93 and EV-B prototypes; the results showed that the three Tibet EV-B93 were always clustered together, confirming that the three strains are closely related
A sero-epidemiology study was performed by using 56 sera samples from healthy children in Tibet; the results showed that the neutralizing antibody titres against EV-B93 were generally lower than those against EV-B81 in a previous study [43] in Tibetan children in 2010: titres of 1:64 accounted for 23.21%, 76.78%, and 0%, respectively, and the geometric mean titer (GMT) was 1:9.94 (Table 4)
The acute flaccid paralysis (AFP) cases caused by polioviruses are less than that caused by the non-polio enteroviruses
Summary
The aim of this study was to characterize the Chinese EV-B93 strain and enrich our knowledge of EV-B93
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.