Abstract
Enterovirus B69 (EV-B69) is a rarely reported type and till date, only the full-length genome sequence of the prototype strain is available. Besides the prototype strain, only limited VP1 sequences of this virus from Africa and India are available in GenBank. In this study, we analyzed the full-length genome sequence of an EV-B69 strain recovered from a patient with acute flaccid paralysis in Niger. Compared with the EV-B69 prototype strain, it had 79.6% and 76.3% nucleotide identity in the complete genome and VP1 coding region, respectively. VP1 sequence analyses revealed also high variation in nucleotide similarity (68.9%–82.8%) with previously isolated EV-B69 strains in India and Africa. The great genetic divergence among EV-B69 strains indicates that this type is not a newly emergent virus, but has circulated for many years at low epidemic strength. Phylogenetic incongruity between structural and non-structural regions and similarity plot analyses revealed that multiple recombination events occurred during its evolution. This study expands the number of EV-B69 whole genome sequences which would help genomic comparison for future studies to understand the biological and pathogenic properties of this virus, assess its potential public health impact and comprehend the role of recombination in the evolution of enteroviruses.
Highlights
Enteroviruses (EVs) are members of the family Picornaviridae, genus Enterovirus and are among the most common human viruses[1]
Enterovirus B69 (EV-B69), and no polioviruses or other enteroviruses were detected from the two samples collected 24 hours apart from the patient
The investigation comprises the isolation of non-polio enteroviruses (NPEVs) strains and their molecular typing based on VP1 sequencing
Summary
Enteroviruses (EVs) are members of the family Picornaviridae, genus Enterovirus and are among the most common human viruses[1]. That the Global Polio Eradication Initiative has brought poliomyelitis cases to the threshold of eradication, non-polio enteroviruses (NPEVs) are considered as one of the associated causes of AFP worldwide. In those countries without EV surveillance systems, such as those from Africa, AFP surveillance turns out to be the only source to study www.nature.com/scientificreports/. We report the identification and whole-genome characterization of an EV-B69 strain (15_491) isolated in 2015 from an AFP patient during polio surveillance activities. This is the first report of EV-B69 in Niger
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