Abstract
Brucella melitensis is an important cause of ovine abortion in several sheep rearing countries, The immunological control of the pathogen in the placenta or by the fetus could be the key to determine the mechanism of abortion and ⁄ or transplacental transmission to the fetus. This work was performed to detect INF gamma gene sequence in the DNA of AL- ousi breed, . and in association with ovine abortion caused by Brucella melitensis. The cytokine gene expression, analyzed by PCR, was performed to explore the immunological regulatory mechanisms of IFN-γ on and its effect on abortion. The results indicated the presence of amplified fragment around ∼340 bp in length compared to the ∼55 bp that was predicted, also, the level of DNA expression was the highest in placenta of normal delivered group and the lowest in aborted placental.
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