Molecular strain typing of Wolbachia infection from Indian mosquitoes using wsp gene

Abstract

ObjectiveTo determine the status of Wolbachia subgroup and phylogenetic relationships in Indian mosquitoes. MethodsRecently we reported Wolbachia infection in eight out of twenty field-caught mosquito species of India, using wsp specific primers. DNA extracted from these mosquito species were used for PCR amplification and sequencing. ResultsWolbachia A harboured in Aedes albopictus and Culex gelidus belongs to the subgroup AlbA whereas Wolbachia B harboured in Aedes albopictus and Culex quinquefasciatus belongs to the subgroup Pip and of Culex vishnui belongs to subgroup Con. However, Wolbachia harboured in Armigeres subalbatus, Armigeres kesseli, Culex sitiens and Toxorhynchites splendens could not be placed into any known subgroup and may represent other unknown strains of Wolbachia. Our phylogenetic analysis revealed eight novel Wolbachia strains, four in the A group and four in the B group. Most of the Wolbachia strains present in Indian mosquitoes belong to the Albo, Pip and Con groups. ConclusionsThe similarities and differences between Wolbachia strains infecting different mosquito species are fundamental for estimating how easily mosquitoes acquire new infections.