Abstract
Blastocystis is one of the most common emerging zoonotic parasites in humans and animals. This study aimed to determine the molecular prevalence and subtype of Blastocystis in cattle (Bos taurus). A total of 150 fresh fecal samples were collected from slaughtered cattle from various slaughterhouses in Kayseri Province, Central Anatolia. Genomic DNA was extracted from all samples and used in PCR analyses of the small subunit ribosomal RNA (SSU rRNA) gene of Blastocystis. Blastocystis positive samples were sequenced for identify subtypes. Obtained sequences were assembled with suitable genetic software, then phylogenetic relationships were revealed. According to PCR analyses, overall prevalence of Blastocystis was determined as 58.7%. The sequence analyses of the PCR product gene revealed the presence of one known livestock-specific subtype, ST10. Phylogenetic analysis revealed that ST10 isolates the characterized in the study were clustered with isolates identified previously from cattle. Molecular characterization and subtype of Blastocystis sp. in slaughtered cattle in slaughterhouses were obtained data with this study.
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