Molecular Phylogeny of Y-Box Proteins and their Cold Shock Domains

Abstract

Purpose: Y-box proteins are a family of highly conserved nucleic acid binding proteins that are conserved from prokaryotes to human. They are supposed to be involved in both transcriptional and translational control. Little is understood about their evolution end genomic diversity among different taxa. The study was performed by combining Bioinformatic and phylogenetic approaches in order to address first cross family evolution of Y-box proteins from different eukaryotic organisms. Methods: For sequence analysis, inferring the phylogenetic tree and evolutionary characterization the protein sequences of Y box proteins from 17 different species were used. The tree was drawn by Neighbour-joining method while using the bootsrapping as a test of inferred phylogeny.Result: The phylogenetic trees were constructed from multiple aligned sequences showing bootstrap values on nodes and species codes on leaves. The analysis of data led to a single most consistent tree. Discussion and conclusion: The results endow with an excellent perception about the evolutionary order of Y-box proteins. The study established an overall framework of information for the family of Y-box proteins, which may facilitate and stimulate the study of this gene family across all organisms.