Abstract
Physids belong to Class Gastropoda; belong to Phylum Mollusca and being bioindicators, intermediate hosts of parasites and pests hold a key position in the ecosystem. There are three species of Genus Physa i.e. P. fontinalis, Physa acuta and P. gyrina water bodies of Central Punjab and were characterized on the basis of molecular markers High level of genetic diversity was revealed by polymorphic RAPD, however SSR markers were not amplified. The multivariate analysis revealed polymorphism ranging from 9.09 percent to 50 percent among the three Physid species. Total number of 79 loci were observed for the three species under study and 24 loci were observed to be polymorphic. These RAPD fragment(s) can be developed into co dominant markers (SCAR) by cloning and can be further sequenced for the development of the Physa species specific markers to identify the introduced and native species in Pakistan.
Highlights
The members of the freshwater family Physidae (Pulmonata: Basommatophora) have invaded the world across all the continents, freshwater lentic habitats except Antarctica
The invasion history of the Physa acuta showed that it was not reported from oriental region (Ebbs et al, 2018), Physids have been reported from Rawalpindi
The study regarding molecular characterization of Physids is the need of time as the cost of management of the invasive species will increase with the time elapse (Simberloff et al, 2013)
Summary
The members of the freshwater family Physidae (Pulmonata: Basommatophora) have invaded the world across all the continents, freshwater lentic habitats except Antarctica These were native to America (Wethington and Lydeard, 2007; Bousset et al, 2014) yet remained unidentified and as a result ignored which shows lesser interest of scientists (Turner & Montgomery, 2009). The invasion history of the Physa acuta showed that it was not reported from oriental region (Ebbs et al, 2018), Physids have been reported from Rawalpindi. (Afshan et al, 2013); Baluchistan (Kakar et al, 2017) and central Punjab (Altaf et al, 2017a, b) in Pakistan This has been recorded as an invasive species in Singapore and Thailand in past few years (Ng et al, 2018). The study regarding molecular characterization of Physids is the need of time as the cost of management of the invasive species will increase with the time elapse (Simberloff et al, 2013)
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