Molecular organization of perineuronal net of the oculomotor neurons in the frog

Abstract

Event Abstract Back to Event Molecular organization of perineuronal net of the oculomotor neurons in the frog Botond Ágoston Gaál1, 2*, Eva Rácz1, Gabor Veres1, Gyorgy Szekely1, 2 and Klara Matesz1, 2 1 Department of Anatomy, University of Debrecen, Hungary 2 University of Debrecen, Hungary The perineuronal net (PN) is a condensation of extracellular matrix macromolecules (ECM) around the perikaryon, proximal dendrites and the axon hillock. It is generally accepted that in mammalian species the major components of the PN are the chondroitin-sulfate proteoglycans (CSPG) that bind to a variety of molecules such as the hyaluronan (HA) and tenascin-R. Since the motoneurons of the brainstem show different response for the axotomy, we suppose that it is associated with different molecular organization of their PN. This study was undertaken to examine the chemical components of the PN around the oculomotor neurons of the frog by immunohistochemical labeling. For identifying neurons, we used Neuron Specific Enolase (NSE) antibody in each reaction, meaning double labeling. Reactions were carried on paraffin embedded sections, treated with St. Marie’s fixative. The HA reaction was positive around the motoneurons showing the strongest intensity. The Wisteria floribunda reaction, the general marker of the CSPG, was negative similarly to the results of the immunohistochemical reaction for the individual PG molecules, i.g. versican and neurocan. Our result suggests that the structure of the PN in the frog brainstem and spinal cord motoneurons is less organized than that of the PN in the vestibular nuclear complex of the frog or in mammalian species. We suppose that in the motoneuronal PN of the frog the function of the CSPG is provided by the phylogenetically ancient heparan-sulfate proteoglycans. This work was supported by OTKA K 67641 and MTA-TKI 242. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Developmental neurobiology and subcortical functions Citation: Gaál B, Rácz E, Veres G, Szekely G and Matesz K (2009). Molecular organization of perineuronal net of the oculomotor neurons in the frog. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.055 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Feb 2009; Published Online: 27 Feb 2009. * Correspondence: Botond Ágoston Gaál, Department of Anatomy, University of Debrecen, Debrecen, Hungary, botond@chondron.anat.dote.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Botond Ágoston Gaál Eva Rácz Gabor Veres Gyorgy Szekely Klara Matesz Google Botond Ágoston Gaál Eva Rácz Gabor Veres Gyorgy Szekely Klara Matesz Google Scholar Botond Ágoston Gaál Eva Rácz Gabor Veres Gyorgy Szekely Klara Matesz PubMed Botond Ágoston Gaál Eva Rácz Gabor Veres Gyorgy Szekely Klara Matesz Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.