Distribution of extracellular matrix macromolecules in the brainstem of rodents

Abstract

Event Abstract Back to Event Distribution of extracellular matrix macromolecules in the brainstem of rodents Adám Deák1*, Ali J. Nikkhah1, Botond Gaál1, Katalin Szakszon2, Tímea Bácskai3 and Klára Matesz1 1 University of Debrecen, Institute of Anatomy, Histology and Embryology, Hungary 2 University of Debrecen, Department of Pediatrics, Hungary 3 University of Debrecen, Hungary The intercellular space of brain is filled with macromolecules of extracellular matrix (ECM). Recent studies hypothesized that ECM has important role in responses of cells to the changes of environment. In our previous study we have demonstrated that the expression of chondroitinsulfate proteoglycans (CSPG), key molecule of ECM, is changing in vestibular nuclei following the injury of vestibular nerve. We intend to investigate whether the expression of CSPG shows similar modification in the brainstem areas that are connected with the vestibular nuclei. In the present study, we have made a detailed ECM-mapping of CSPG in order to compare the changes of expression pattern. Experiments were performed on adult mice and rats. On anesthetized animals the brainstem was removed and immersed into Sainte-Marie’s fixative. The paraffin embedded specimens were cut in transverse and sagittal plane and biotinylated Wisteria floribunda agglutintin (WFA) was applied to detect the CSPG. The WFA reaction showed an area–dependent distribution in the gray and white matter of brainstem. In the gray matter, the WFA positivity displayed a homogenous or fine granular appearance in the neuropil. The perikarya and dendrites were surrounded with a narrow area representing the perineuronal net (PN). In the mesencephalon, the WFA reaction was intensive in the eye moving motor nuclei, red nucleus and substantia nigra. In the rhombencephalon, each somatomotor cranial nerve nucleus displayed moderate or strong reaction. The PN was intensively stained in the magnocellular nucleus of reticular formation, trapezoid body and the inferior olive. The white matter showed a very intensive reaction in the cerebellar peduncle. The heterogeneous distribution of CSPG in the neural network of brainstem underlying the gaze and posture control may indicate their different involvement in the restoration of vestibular function following the nerve injury. This work was supported by OTKA K 67641 and MTA-TKI 242. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Sensory and motor systems Citation: Deák A, Nikkhah AJ, Gaál B, Szakszon K, Bácskai T and Matesz K (2010). Distribution of extracellular matrix macromolecules in the brainstem of rodents. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00156 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 30 Apr 2010; Published Online: 30 Apr 2010. * Correspondence: Adám Deák, University of Debrecen, Institute of Anatomy, Histology and Embryology, Debrecen, Hungary, adam@chondron.anat.dote.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Adám Deák Ali J Nikkhah Botond Gaál Katalin Szakszon Tímea Bácskai Klára Matesz Google Adám Deák Ali J Nikkhah Botond Gaál Katalin Szakszon Tímea Bácskai Klára Matesz Google Scholar Adám Deák Ali J Nikkhah Botond Gaál Katalin Szakszon Tímea Bácskai Klára Matesz PubMed Adám Deák Ali J Nikkhah Botond Gaál Katalin Szakszon Tímea Bácskai Klára Matesz Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.