Abstract
(R,R)ZX-5 is a NO regulatory compound, which could significantly increase choroidal blood flow in New Zealand rabbit. The aim of this paper is to investigate the molecular mechanism of (R,R)ZX-5 promoting NO production. Besides this, we also investigated the antiangiogenic activity of (R,R)ZX-5. Analysis of Western blot showed that (R,R)ZX-5 up-regulated the expression of Akt, p-Akt (Thr473), eNOS and p-eNOS (Ser1177), down-regulated the expression of Cyclin D1 in human retinal endothelial cells and escalated the intracellular free Ca2+ concentration. Additionally, (R,R)ZX-5 inhibited the growth of blood vessels in the chick chorioallantoic membrane model. It is concluded that (R,R)ZX-5 promotes choroidal blood flow through PI3K/Akt-eNOS and Akt-Ca2+-eNOS pathways. Additionally, (R,R)ZX-5 can inhibit angiogenesis.
Highlights
Age-related macular degeneration (AMD) is an eye condition that leads to the deterioration of the center of the retina, called the macula, leading to loss of central vision
In order to clarify the molecular mechanism of (R,R)ZX-5 promoting Nitric oxide (NO) production, we investigated and found that the compound can increase the expression of Endothelial nitric oxide synthase (eNOS) and NO release in human umbilical vein endothelial cells [7]
We investigated to see if ZX-5 increases NO release through PI3K/Akt/eNOS/NO pathway or PI3K/Akt/Ca2+/eNOS/NO pathway
Summary
Age-related macular degeneration (AMD) is an eye condition that leads to the deterioration of the center of the retina, called the macula, leading to loss of central vision. Nitric oxide (NO) production by vascular endothelium is important in the regulation of blood flow. Pathological studies found that expression of eNOS was reduced in the choroidal blood vessels and choroidal vascular endothelial cells in patients with AMD [5]. Found that compound (R,R)ZX-5 could significantly increase choroidal blood flow in New Zealand rabbit, but did not affect the blood flow in iris and ciliary body. (R,R)ZX-5 has been proven to improve the choroidal blood flow without affecting the sclera and ciliary bodies in New Zealand white rabbits via increasing NO production [6]. In order to clarify the molecular mechanism of (R,R)ZX-5 promoting NO production, we investigated and found that the compound can increase the expression of eNOS and NO release in human umbilical vein endothelial cells [7]. The main advantages of CAM angiogenesis assays are their low cost, simplicity, reproducibility, and reliability [9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
