Molecular mechanisms of bone reconstruction in new dental implant technology

Abstract

Abstract Aim. In this work was investigated the potential role of bone remodeling cells (osteoblasts and osteoclasts) in in a new technic of dental implant. Peripheral blood (PB) in steady state was collected at apparent health patients from plasma for to follow a new technique of dental implant, having the theoretical source medical literature from Department of Cell Biology and Neuroscience from different countries. Material and Method. A total of 10 patients (8 males and 2 females), between 50 and 65 years (mean age 57.5 years) were appropriately informed and after that them were collected a small volume of blood, 20 cm ³, drown from peripheral vein, using sodium citrate as anticoagulant, 4.5 mL in 4 coagulation tubes of 5 ml capacity, (38% sodium citrate as an anticoagulant, 0.5 ml). The system for to prepare samples have used, a centrifuge technique, at low-speed (280G), over a period of 8 minutes, at ambient temperature. For activation and aggregation of platelets, 50 ml of calcium chloride 10% was used for each 1cc of preparation reach in growth factor (PRGF). After the blood sample had been centrifuged and plasma had been separated from red and white globules, the plasma in each tube was fractionated into four distinct fractions seated on a layer of white blood cells and containing a considerable amount of platelet GFs to which were added dental bone powder filings. The plasma delivered in biochemistry dry vacuetts, were kipped for time a 30 minutes, in a thermostat at 37 C*, for to obtain auto-homolougous fibrin for to be used in seal at post-extraction site Results. The patients, after 24 weeks from extraction with the alveolus sealing, made the CT scan for the evaluation of regenerated new bone density and all dental implants was resolved. Conclusion. These results provide strong evidence for a sequential physiologic mechanism through which the molecular mechanisms gain access to active TGF-s and renew dental bone.