Molecular mechanism of IL17A-IL17F involved in children with allergic rhinitis through IL17RC-IL33-NF-kB signaling axis

Abstract

Objective: Allergic rhinitis (AR) is a common chronic nasal mucosal congestion disease of children, and its pathogenesis is associated with immune factors. Methods: 50 cases of children were collected and their nasal mucus was used to detect inflammatory factors IL-17A, IL-17F and IL-33 level, as well as the proportion of ILC2 and Th2 in blood labeled by flow cytometry. In addition, the allergic rhinitis model of immature mice was established. HE staining was used to observe nasal mucosa. IgE, IL-17A, IL-17F and IL-33 levels were detected, and the ratio of ILC2 and Th2 in blood was marked by flow cytometry. The expressions of IL17-RC, TRAF6, NF-kBp65 and MAPK protein in IL17RC-IL33-NF-kB signal pathway were measured by western blot. Results: The results indicated that IL-17A, IL-17F and IL-33 were significantly higher in children with allergic rhinitis and young model mice than that in control group. The content of CD4+IL-4+subgroup in Th2 in blood of model mice was high. The same trend as CD127+CD117+CRTH2+subgroup in ILC2. HE staining showed that the nasal mucosa of mice was intact in the control group, but the nasal mucosa epithelium of mice in the model group was destroyed. Conclusion: IL17-RC, TRAF6, NF-kBp65 and MAPK in nasal mucosal of model mice showed high expression, confirming that inflammatory factor IL17A-IL17F activated IL33 transcription through IL17RC and Activated ILC2 and Th2 cells involving in allergic inflammatory responses.