Abstract
To observe the impact of Yupingfengsan Oral Liquid on the expression of IL-4 and IFN-γ in AR mice’s serum and expression level of nuclear factor—κB (NF-κB) protein and gene in nasal mucosa. Method: Forty BALB/c mice were randomly divided into the normal group, model group, Yupingfengsan Oral Liquid group (6 g/kg) and Loratadine group, with 10 mice per group. AR mice model was established by OVA, and IL-4 and IFN-γ contents can be measured with ELISA. The morphological changes of nasal mucosa were observed by hematoxylin eosin (HE) staining and NF-κB expression in the nasal mucosa of mice was tested with Real-Time PCR and Western blot. Results: Compared with the model group, the nasal symptoms in the Yupingfengsan Oral Liquid group and Loratadine group were obviously relieved. HE staining showed that there was a little inflammatory cell infiltration in the nasal mucosa of Yupingfengsan Oral Liquid group and Loratadine group and it was significantly reduced when compared with the model group. IL-4 level in the serum and expression of NF-κB protein and gene in the nasal mucosa was consistent and it was decreased when compared with the model group (P γ level in the serum was increased (P κB expression, balance the percentage of Th1/Th2 cells, increase the IFN-γ level in the serum and decrease the IL-4 level.
Highlights
Allergic rhinitis (AR) is a common and frequently-occurring disease in the otorhinolaryngology head and neck surgery department, and it is a type-I allergic disease resealed by the IgE-mediated mediators, and involved with multiple immune cells and cytokines after the body is exposed to the allergen [1]
The result showed that except the normal group, the mice in the model group, Yupinfengsan Oral Liquid group and loratadine group suffered from AR symptoms with the symptom score over 5 points
The recent researches has shown that Yupingfengsan can give the role of immune regulation, anti-inflammatory, antibacterial and other effects via different mechanisms, and it can prevent and treat the allergic rhinitis, upper respiratory tract infection and other diseases
Summary
Allergic rhinitis (AR) is a common and frequently-occurring disease in the otorhinolaryngology head and neck surgery department, and it is a type-I allergic disease resealed by the IgE-mediated mediators, and involved with multiple immune cells and cytokines after the body is exposed to the allergen [1]. Its pathogenesis is complex and is not clear, wherein, the balance disorder of Th1/Th2 cytokines is the key for the development of allergic diseases [2]. The studies have shown that NF-κB can adjust the release of Th cytokine and interfere the balance of Th1/Th2 cytokine after activation, so as to be involved in the occurrence and development of immunity and inflammation [3]. Yupinfengsan Oral Liquid, which is originated from Yupinfengsan, is a classic prescription for reinforcing the vital energy and consolidating the constitution, and it is functioned with treatment, prevention and good immune regulation [4]. By establishing the mice AR model and detecting the cytokine contents of IL-4 and IFN-γ in serum, and NF-κB protein and gene expression level in the nasal mucosa, this experiment discusses the regulatory role of Yupinfengsan Oral Liquid on the expression of related cytokines of the mice AR model
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