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Abstract
Key messageWe report molecular mapping and inheritance of restoration of fertility (Rf) in A4 hybrid system in pigeonpea. We have also developed PCR-based markers amenable to low-cost genotyping to identify fertility restorer lines.Commercial hybrids in pigeonpea are based on A4 cytoplasmic male sterility (CMS) system, and their fertility restoration is one of the key prerequisites for breeding. In this context, an effort has been made to understand the genetics and identify quantitative trait loci (QTL) associated with restoration of fertility (Rf). One F2 population was developed by crossing CMS line (ICPA 2039) with fertility restorer line (ICPL 87119). Genetic analysis has shown involvement of two dominant genes in regulation of restoration of fertility. In parallel, the genotyping-by-sequencing (GBS) approach has generated ~ 33 Gb data on the F2 population. GBS data have provided 2457 single nucleotide polymorphism (SNPs) segregating across the mapping population. Based on these genotyping data, a genetic map has been developed with 306 SNPs covering a total length 981.9 cM. Further QTL analysis has provided the region flanked by S8_7664779 and S8_6474381 on CcLG08 harboured major QTL explained up to 28.5% phenotypic variation. Subsequently, sequence information within the major QTLs was compared between the maintainer and the restorer lines. From this sequence information, we have developed two PCR-based markers for identification of restorer lines from non-restorer lines and validated them on parental lines of hybrids as well as on another F2 mapping population. The results obtained in this study are expected to enhance the efficiency of selection for the identification of restorer lines in hybrid breeding and may reduce traditional time-consuming phenotyping activities.
Highlights
Pigeonpea [Cajanus cajan (L.) Millspaugh] is an often cross-pollinating legume crop grown in tropical and subtropical regions of the world
single nucleotide polymorphism (SNPs) genotyping data collected as mentioned above was used for linkage analysis using Joinmap version 4.0 (Van Ooijen and Voorrips 2006) following “Regression mapping algorithm”
Linkage groups in genetic map were named according to pseudomolecules defined in the draft genome sequence of pigeonpea (Varshney et al 2012)
Summary
Pigeonpea [Cajanus cajan (L.) Millspaugh] is an often cross-pollinating legume crop grown in tropical and subtropical regions of the world. It is commonly grown in Communicated by Matthew N Nelson. University of Western Australia, Crawley, WA, Australia rainfed marginal lands as intercrops at subsistence level This crop has good buffering ability against various biotic and abiotic stresses and produce reasonable amounts of yields in unfavourable conditions frequently occurring in this area makes it very useful for low-input farming systems. The hybrid seeds production is based on sporophytic cytoplasmic male sterility (CMS) system; it is known as “three line hybrid system”. A number of reports on CMS development from Cajanus species have been published (Ariyanayagam et al 1995; Reddy and Faris 1981; Saxena and Kumar 2003; Wanjari and Patel 2003; Saxena et al 2005; Mallikarjuna and Saxena 2005; Mallikarjuna et al. Vol.:(0123456789)
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