Abstract
BackgroundAneuploidy, a condition caused by an imbalance between the relative dosages of chromosomes, generally produces a novel phenotype specific to the molecular karyotype. Few techniques are currently available for detecting the molecular karyotypes of aneuploids in plants.ResultsBased on this imbalance in chromosome dosage, a new approach (referred to as ‘SSR-qPCR’) combining simple sequence repeat (SSR) markers and quantitative real-time PCR (qPCR) has been developed and utilized to detect some common aneuploids irrespective of heterozygosity. We screened 17 specific SSR markers covering all loquat linkage groups and redesigned 6 pairs of primers for SSR markers that can detect loquat chromosome aneuploidies. The SSR-qPCR detection results obtained for hybrid progeny and open-pollination progeny of triploid loquat showed diagnostic accuracies of 88.9% and 62.5%, respectively, compared with the chromosome preparation results.ConclusionSSR-qPCR can detect loquat aneuploids and be used to construct the entire molecular karyotypes of aneuploid individuals. Therefore, this method offers a novel alternative for the detection of chromosome aneuploidies.
Highlights
Aneuploidy, a condition caused by an imbalance between the relative dosages of chromosomes, generally produces a novel phenotype specific to the molecular karyotype
If there are appropriate simple sequence repeat (SSR) markers in each linkage group (LG) used for Quantitative real-time polymerase chain reaction (qPCR), accurate detection of aneuploidy based on the whole-chromosome dosage can be achieved through the use of qPCR combined with SSR markers to distinguish aneuploidy irrespective of heterozygosity
Screening of 17 SSR markers covering all loquat LGs by qPCR We aimed to develop a rapid and reliable method for studying aneuploid karyotypic abnormalities
Summary
Aneuploidy, a condition caused by an imbalance between the relative dosages of chromosomes, generally produces a novel phenotype specific to the molecular karyotype. Few techniques are currently available for detecting the molecular karyotypes of aneuploids in plants. The details of polyploidization have been described in Malus, with particular emphasis on aneuploidy, via detection of the types of heterozygous genotypes and the corresponding frequencies of occurrence [12]. Both of these methods are limited to heterozygous genotypes; in particular, the detection of the occurrence frequencies of heterozygous genotypes using statistical methods requires large numbers of codominant SSR markers. With the publication of the genome sequences of major crops, more SSR markers have become available
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